seqviz

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SeqViz

SeqViz is a sequence viewer. It supports multiple input formats, display settings, and callbacks for integration into any JavaScript app.

• Features
• Usage
  • Installation
  • Instantiation
  • Props
  • Viewer without React
• Demo
• Contact Us

Features

Multiple input formats

• Raw sequence and annotations
• File (FASTA, GenBank, SBOL, SnapGene)
• Accession (NCBI or iGEM)

Linear and/or Circular sequence viewer

• Display as a linear viewer, circular viewer, or both
• Annotations with names and colors
• Amino acid translations
• Enzyme cut sites
• Searching and highlighting

Sequence and element selection

• Selecting a sequence range -- or clicking an annotation, translation, enzyme or searchElement -- will highlight that section of the viewer(s) and pass the selection to the onSelection() callback

Usage

Installation

npm

npm install seqviz

CDN

<script src="https://unpkg.com/seqviz"></script>

Instantiation

React

import { SeqViz } from "seqviz";

export default () => (
  <SeqViz
    name="J23100"
    seq="TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC"
    annotations={[{ name: "promoter", start: 0, end: 34, direction: 1, color: "blue" }]}
  />
);

Non-React

More details are in the Viewer without React section.

<script>
  window.seqviz
    .Viewer("root", {
      name: "L09136",
      seq: "tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgca",
      style: { height: "100vh", width: "100vw" },
    })
    .render();
</script>

Props

All the following are usable as props for the React component (seqviz.SeqViz) or as options for the plain JS implementation (seqviz.Viewer()).

Required (one of)

seq (='')

A sequence to render. Can be DNA, RNA, or an amino acid sequence.

accession (='')

An NCBI accession ID or iGEM part ID. Populates name, seq, and annotations.

file (=null)

A File, Blob, or body (string/utf8) from a FASTA, Genbank, SnapGene, or SBOL file. Populates name, seq, and annotations.

Optional

viewer (='both')

One of "linear" | "circular" | "both" | "both_flip". The type and orientation of the sequence viewers. both means the circular viewer fills the left side of SeqViz and the linear viewer fills the right. both_flip is the opposite: the linear viewer is on the left and the circular viewer is on the right.

name (='')

The name of the sequence/plasmid. Shown at the center of the circular viewer.

compSeq (='')

The complement sequence. Inferred from seq by default.

annotations (=[])

An array of annotations to show. Each Annotation requires 0-based start (inclusive) and end (exclusive) indexes. For arrows, set the annotation's direction to 1 for forward arrows and -1 for reverse arrows. Names are rendered on top of the annotation.

annotations = [
  { start: 0, end: 22, name: "Strong promoter", direction: 1 }, // [0, 22)
  { start: 23, end: 273, name: "GFP" },
  { start: 300, end: 325, name: "Weak promoter", direction: -1, color: "#FAA887" },
];

In the example above, the "Strong promoter" would span the first to twenty-second basepair.

translations (=[])

An array of translations showing amino acids beneath a DNA sequence. Requires 0-based start (inclusive) and end (exclusive) indexes relative the DNA sequence. A direction is required: 1 (FWD) or -1 (REV).

translations = [
  { start: 0, end: 90, direction: 1 }, // [0, 90)
  { start: 191, end: 522, direction: -1 },
];

enzymes (=[])

An array of restriction enzymes to show the recognition sites for. A list of pre-defined enzymes in src/utils/enzymes.ts can be referenced by name. Example:

enzymes = [
  "EcoRI",
  "PstI",
  {
    name: "Cas9",
    rseq: "NGG", // recognition sequence
    fcut: 0, // cut index on FWD strand, relative to start of rseq
    rcut: 1, // cut index on REV strand, relative to start of rseq
    color: "#D7E5F0", // color to highlight recognition site with
  },
];

Enzymes can be limited to a range via the range property on an Enzyme object:

enzymes = [
  {
    name: "Cas9",
    rseq: "NGG", // recognition sequence
    fcut: 0, // cut index on FWD strand, relative to start of rseq
    rcut: 1, // cut index on REV strand, relative to start of rseq
    color: "#D7E5F0", // color to highlight recognition site with
    // only show recognition sites between 100th and 250th index [100,250]
    range: {
      start: 100,
      end: 250,
    },
  },
];

highlights (=[])

Ranges of the sequence to highlight. A default color from colors is used if none is provided.

highlights = [
  { start: 36, end: 66, color: "magenta" },
  { start: 70, end: 80 },
];

zoom (={ linear: 50, circular: 0 })

How zoomed the viewer(s) should be 0-100. Key'ed by viewer type (viewer).

colors (=[])

An array of colors to use for annotations, translations, and highlights. Defaults to:

colors = [
  "#9DEAED", // cyan
  "#8FDE8C", // green
  "#CFF283", // light green
  "#8CDEBD", // teal
  "#F0A3CE", // pink
  "#F7C672", // orange
  "#F07F7F", // red
  "#FAA887", // red-orange
  "#F099F7", // magenta
  "#C59CFF", // purple
  "#6B81FF", // blue
  "#85A6FF", // light blue
];

bpColors (={})

An object that maps basepairs to their color. The key/bp is either a nucleotide type or 0-based index. Example:

bpColors = { A: "#FF0000", T: "blue", 12: "#00FFFF" };

style (={})

Style for seqviz's outer container div. Empty by default. Useful for setting the height and width of the viewer if the element around seqviz lacks a defined height and/or width. For example:

style = { height: "100vh", width: "100vw" };

onSelection (=null)

Callback function executed after selection events. Should accept a single selection argument: (selection) => {}.

This occurs after drag/drop selection and clicks. If an annotation, translation, enzyme or searchElement was clicked, the selection object will have info on the selected element. The example below is of a selection object following an annotation click.

{
  // selection
  "name": "lacZ fragment",
  "type": "ANNOTATION",
  "seq": "ctatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgccaagcttgcatgcctgcaggtcgactctagaggatccccgggtaccgagctcgaattcgtaatcatggtcat",
  "gc": 55.3,
  "tm": 85,
  "start": 133,
  "end": 457,
  "length": 324,
  "direction": -1
  "clockwise": true,
  "color": "#8FDE8C",
}

search (=null)

A search object for specifying search results to highlight on the viewer. An example is below:

search = { query: "aatggtctc", mismatch: 1 };

Searching supports the following nucleotide wildcards within the query.

{
  "y": ["c", "t"],
  "r": ["a", "g"],
  "w": ["a", "t"],
  "s": ["c", "g"],
  "k": ["g", "t"],
  "m": ["a", "c"],
  "d": ["a", "g", "t"],
  "v": ["a", "c", "g"],
  "h": ["a", "c", "t"],
  "b": ["c", "g", "t"],
  "x": ["a", "c", "g", "t"],
  "n": ["a", "c", "g", "t"]
}

mismatch is an int denoting the maximum allowable mismatch between the query and a match within the viewer's sequence (see: Hamming distance).

onSearch (=null)

Callback executed after a search event with a searchResults object. Called once on initial render. An example of searchResults is below:

[
  {
    start: 728,
    end: 733,
    direction: 1,
    index: 0,
  },
  {
    start: 1788,
    end: 1793,
    direction: -1,
    index: 1,
  },
];

copyEvent (=(KeyboardEvent) => false)

A functions that returns whether to copy the selected range on the viewer(s) to the user's clipboard.

An example of an copyEvent function for copying after ctrl+c or meta+c events:

copyEvent = event => event.key === "c" && (event.metaKey || event.ctrlKey);

showComplement (=true)

Whether to show the complement sequence.

showIndex (=true)

Whether to show the index line and ticks below the sequence.

rotateOnScroll (=true)

The circular viewer rotates when scrolling over the viewer by default. That can be disabled with rotateOnScroll: false.

backbone (='')

This is a feature specific to BioBricks (accession). The library currently supports BBa_K1362091, BBa_K823055, pSB1A3, pSB1A7, pSB1AC3, pSB1AK3, pSB1AT3, pSB1C3, pSB1K3, and pSB1T3.

Custom backbones, as DNA strings, are also supported (for example: ATGATATAGAT).

Viewer without React

For usability in non-React apps, we provide a thin wrapper around the React component. The viewer's constructor accepts two arguments:

• element: either an element id or an element, e.g. from document.getElementById()
• props: props as documented above

const element = document.getElementById("root");
const viewer = seqviz.Viewer(element, props);
// Render the viewer to the DOM at the node passed in $element`.
viewer.render();
// To later update the viewer's configuration and re-renders.
viewer.setState(props);
// To render the viewer, eg for server-side rendering, and returns it as an HTML string.
viewer.renderToString();

Demo

You can see a demonstration with iGEM BioBricks at: tools.latticeautomation.com/seqviz.

For developers, the demo source code is at seqviz/demo.

You can also check out an example of using SeqViz to view NCBI GenBank entries in our Medium post.

Contact Us

This library is maintained by Lattice Automation.

You can report bugs and request features at Issues, or contact us directly at [email protected]