Rasmus Kirkegaard

icon indicating a website link http:/dk.linkedin.com/in/rasmuskirkegaard/

icon company University of Aalborg icon location Aalborg icon location Currently employed as a staff scientist at AAU working on generating high quality genomes from various environments using @nanoporetech DNA sequencing

Results 9 comments of Rasmus Kirkegaard

Option for pacbio CCS/HiFi reads

Yeah I think Heng Li has thought this through minimap2 -ax asm20 ref.fa pacbio-ccs.fq.gz > aln.sam # PacBio CCS genomic reads (https://github.com/lh3/minimap2) Me neither but I assume with the recent...

Option for pacbio CCS/HiFi reads

Maybe just add the full suite of minimap2 options? It would be nice to be able to run minipolish with short reads as well :D.

bonito basecaller to have a resume mode

Hi Chris Thanks. That is a nice workaround. Best Rasmus

Add information about plasmid annotation

How big a fraction of these still has no known function?

Client 1 has timed out.

Hi @marcus1487 I tried running megalodon with the remora model and it ran without this issue. So it could indicate that it is a rerio model related issue? Any chance...

Expected release of all-context methylation models for kit 12?

Hi Marcus That sounds great. Really looking forward to it. Best regards Rasmus

Print Mechanical Z-stage without support or add supports?

Hi I am surprised how well this printed even though there are quite a few horizontal pieces that have to be printed in the air. Best regards Rasmus ![IMG_20210601_151221](https://user-images.githubusercontent.com/4373417/120436708-8dab2a80-c37f-11eb-8e31-5df8330000ac.jpg)

CUDA

Hi Will you consider adding that to the mainpage of this repository?

Finding overlaps with minimap... Killed

Tried running the long read assembly outside unicycler? minimap2 seems to produce better assemblies than minimap and is currently not supported by unicycler. So you might get a long way...