JiaZhong28

 i was running megahit for contig assemblely with big data(179G for r1.fq) under the command:"megahit -1 r1.fastq -2 r2.fastq --min-count 2 --k-min 27 --k-max 87 --k-step 10 --num-cpu-threads 20...

Hi,Stephen:   How do you confirm than a gene from a OTU is a single-gene when you ues  PhyEco  database by hmmscan.What do you set up any cut-off? best YYY