José Carlos Montañés

Sorry for not completmenting the thread with the pasa logfile the last lines of the pasa-assembly.log show the following: ``` [Tue Jul 26 10:48:19 2022] Running CMD: /soft/EB_repo/devel/programs/noarch/miniconda3/2022-05/envs/funannotate/opt/pasa-2.4.1/scripts/assign_clusters_by_stringent_alignment_overlap.dbi -M Saccharomyces_paradoxus_pasa...

Hi Jon, That was my thinking that /tmp maybe can not store the amount of data that I am providing. The issue is that some species work but others it...

Addendum: I noticed that some of my genomes are hard masked. Curiously, those genomes are the ones that are taking the longest and causing the most problems. Do you think...

Hi again, I am writing this for the record. I recommend assembling long nanopore reads because produce better results (at least the UTR regions) and takes less time. That solved...

I am having the same issue. I am doing a saturation analysis subsampling a bam file and when I use RNA-seq data with 4,680,970 reads it seems that there are...