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While using hap.py, there is a problem:

Open pioneer-pi opened this issue 1 year ago • 0 comments

I use docker to start hap.py. The docker sentences:

docker run -it \
  -v "${PWD}":"/input" \
  -v "${PWD}/my_output:/output" \
  jmcdani20/hap.py:v0.3.12 /opt/hap.py/bin/hap.py \
  /input/chr20.vcf.gz \
  /output/my.vcf \
  -f "/input/HG001_GRCh37_GIAB_highconf_CG-IllFB-IllGATKHC-Ion-10X-SOLID_CHROM1-X_v.3.3.2_highconf_nosomaticdel_chr.bed" \
  -r "/input/ucsc_hg19.fa" \
  -o "/output/happy.output" \
  --engine=vcfeval \
  --pass-only \
  -l chr20

The error information is :

2024-07-13 12:29:32,677 WARNING  Creating template for vcfeval. You can speed this up by supplying a SDF template that corresponds to /input/ucsc_hg19.fa
2024-07-13 12:30:43,939 ERROR    Error running rtg tools / vcfeval. Return code was 1, output:  / null
Error: RTG has encountered a difficulty, please contact [email protected]
Please send the log file ('/tmp/vcfeval.result3BAEs0.dir/vcfeval.log') to Real Time Genomics at [email protected]
 

2024-07-13 12:30:43,939 ERROR    Traceback (most recent call last):
2024-07-13 12:30:43,939 ERROR      File "/opt/hap.py/bin/hap.py", line 529, in <module>
2024-07-13 12:30:43,939 ERROR        main()
2024-07-13 12:30:43,939 ERROR      File "/opt/hap.py/bin/hap.py", line 473, in main
2024-07-13 12:30:43,939 ERROR        tempfiles += Haplo.vcfeval.runVCFEval(args.vcf1, args.vcf2, output_name, args)
2024-07-13 12:30:43,939 ERROR      File "/opt/hap.py/lib/python27/Haplo/vcfeval.py", line 132, in runVCFEval
2024-07-13 12:30:43,940 ERROR        raise Exception("Error running rtg tools / vcfeval. Return code was %i, output: %s / %s \n" % (rc, o, e))
2024-07-13 12:30:43,940 ERROR    Exception: Error running rtg tools / vcfeval. Return code was 1, output:  / nullError: RTG has encountered a difficulty, please contact [email protected] send the log file ('/tmp/vcfeval.result3BAEs0.dir/vcfeval.log') to Real Time Genomics at [email protected]

pioneer-pi avatar Jul 13 '24 12:07 pioneer-pi