Don't find the reference

[kangxiongbin]

CODE:

hap.py 40X_deepvariant_illumina_tumor_only.vcf 40X_PEPPER_MARGIN_tumor_only.vcf -r /public/groups/vg/xkang12/data/GCA_000001405.15_GRCh38_no_alt_analysis_set.fasta -o hap_result/

ISSUE:

WARNING No reference file found at default locations. You can set the environment variable 'HGREF' or 'HG19' to point to a suitable Fasta file. Hap.py 2022-07-26 17:27:00,351 ERROR The output path should specify a file name prefix. Please specify a valid output path and prefix using -o. For example, -o /tmp/test will create files named /tmp/test* . 2022-07-26 17:27:00,352 ERROR Traceback (most recent call last): 2022-07-26 17:27:00,352 ERROR File "/public/home/xkang12/software/miniconda3/envs/py2/bin/hap.py", line 540, in 2022-07-26 17:27:00,352 ERROR main() 2022-07-26 17:27:00,352 ERROR File "/public/home/xkang12/software/miniconda3/envs/py2/bin/hap.py", line 225, in main 2022-07-26 17:27:00,352 ERROR raise Exception("The output path should specify a file name prefix. Please specify a valid output path " 2022-07-26 17:27:00,352 ERROR Exception: The output path should specify a file name prefix. Please specify a valid output path and prefix using -o. For example, -o /tmp/test will create files named /tmp/test* .

[b-math]

Hi, you can set the path to your reference genome fasta file (replace "$ref_genome_path" by your path): export HGREF=$ref_genome_path If you run your command afterwards, it should work. There is also the possibility to save your reference genome in a default location, so you dont have to set the path anew in each new bash. But I don't recall the folder right now.

Best regards Barbara