Which model to use for r9.4.1 fast calls?

[sagnikbanerjee15]

Hello,

I am unsure of the model I should use for Minion r9.4.1 where the base calling was performed with dna_r9.4.1_450bps_fast.cfg

I saw a "fast" compatible model for r10.4 from rerio but not for r9.4.1

Could you please suggest the best model?

Thank you

[aquaskyline]

All HKU-provided ONT models at https://github.com/HKU-BAL/Clair3#hku-provided-models are for r9.4.1. Please check the one that matches the Guppy version you used.

[sagnikbanerjee15]

Hi,

Thanks for the quick reply. Among the models that have been mentioned, none are for the "fast" base calling method. The models are for either "hac" or "sup".

Thanks.

[aquaskyline]

You can use a SUP model on fast base-called data. But not the reverse.

[sagnikbanerjee15]

Oh good to know. Thanks!

[FAFUshiyan]

Hi， I am using model dna_r9.4.1_450bps_fast_prom.cfg for basecalling, Guppy's version is Version 4.0.11, which would be the best choice of model for me? Is r941_prom_hac_g360+g422.tar.gz the best choice?

[zhengzhenxian]

Hi,

Yes, the r941_prom_hac_g360+g422 model is recommended for Guppy3 and Guppy4 data.

[FAFUshiyan]

Hi,

Yes, the r941_prom_hac_g360+g422 model is recommended for Guppy3 and Guppy4 data.

Thank you for your quick reply.