DepledgeLab

understanding modified bases threshold setting in Dorado

2

# Issue Report I'm using Dorado v0.7.0 for all context m6A calling but I am uncertain how to interpret the --modified-based-threshold paramater. _--modified-bases-threshold the minimum predicted methylation probability for a...

Setting appropriate thresholds for calling m6A and pseU using modkit pileup - What to make of high Nfail numbers?

1

Hello, After spending a significant amount of time going through the various modkit threads on how best to set the mod-thresholds and filter-thresholds values, I am still uncertain as to...