Oscar Wilkins comments

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                                            Oscar Wilkins

Very few duplex reads with short amplicons

@vellamike Do you have any info on when duplex pairing for short amplicons might be ready? Thanks!

Alignment of duplex reads is incorrect or truncated in some cases, giving truncated or poor quality reads?

[example.zip](https://github.com/nanoporetech/dorado/files/13190862/example.zip) I attach the pod5 and bam file (default parameters, super accuracy 4khz [email protected]) for the example above

Alignment of duplex reads is incorrect or truncated in some cases, giving truncated or poor quality reads?

Oh that's great news, looking forward to it. In this case they are definitely true positive (experiment was designed to ensure this) so hopefully the updates you are releasing fix...

Alignment of duplex reads is incorrect or truncated in some cases, giving truncated or poor quality reads?

Unfortunately I don't see an improvement here

Alignment of duplex reads is incorrect or truncated in some cases, giving truncated or poor quality reads?

Thanks! Just to note that the two reads in the .pod5 file have been split by some custom software. So some of the metadata values have been imputed. The actual...

Support for RNA004?

Thanks James. Yes, I can see why this is a difficult project to prioritise given the situation with nanopore. I actually contacted a rep to ask when barcoding is likely...