Deco313

Hi, I tried again using Spades 3.15.4 and got the same error. Command line: /ichec/work/nglif049b/SPAdes-3.15.5-Linux/bin/spades.py -o /ichec/work/nglif049b/Mitogenomes/cleaned/BAM001 -1 /ichec/work/nglif049b/Mitogenomes/raw/BAM001/BAM001-READ1.fq.gz -2 /ichec/work/nglif049b/Mitogenomes/raw/BAM001/BAM001-READ2.fq.gz --memory 50 --cov-cutoff 2 --careful --threads 15 --phred-offset 33...

Hi so it is an issue with RAM, see below the response from my system administrator. I had specified 20 threads, so would that be the 20 instances of spades-hammer....

My question exactly This was just the specified threads. Should I lower the threads just to one? On Wed 29 Mar 2023, 14:48 Anton Korobeynikov, ***@***.***> wrote: > I had...

And I do appreciate your help. My system administrator wanted to know why my code would run 20 instances of spades hammer. By threads, I mean the --threads command in...

yes, I have tried increasing and decreasing the number of threads, but it still gets caught. So for the time, I tried it on a high memory node with 1.5...