ChloePZS

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Thanks a lot for your quick reply! The process doesn't do any swapping and we haven't use any cluster either. The computer I am using has 12 cores (2.4GHz) and...

I checked the presence of primers + additional bases before & after Cutadapt Exple for a sample in FWD ![image](https://user-images.githubusercontent.com/47737491/193017579-d05c7243-0fff-42dd-9d10-b558ff506785.png) Cutadapt removes the primers and everything before, though I noticed...

Thanks a lot for coming back to my issue. plotComplexity (after cutadapting & filtering) gives me something quite good I think : ![image](https://user-images.githubusercontent.com/47737491/194497791-8cc49e18-c96c-47dd-9b6d-a7bb5bcd6690.png) ![image](https://user-images.githubusercontent.com/47737491/194498027-dcb6f604-7855-49e6-928a-58d358e6281b.png) Would you still add "rm.lowcomplex" during...

Hi Benjamin, I ran the different functions for error rates estimation and the best plots were obtained when altering loess arguments (weights and span) & enforcing monotonicity (below for FWD...

I'll go ahead and do that then ! Many thanks again for all your insights, it's been very helpful.