ChenManqi2

 For custom references or references missing the promoter.bed file, the count of promoter_region_fragments is 0. I use TSS_fragments to filter cell barcodes instead.

I have met the error too. I solved this problem after invoking the function 'plot_genes_in_pseudotime' with code 'monocle3::plot_genes_in_pseudotime()'. It is likely that you invoked the 'plot_genes_in_pseudotime' from monole rather than...