CDieterich comments

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                                            CDieterich

reads did not aligned to 3' end

Same problem over here for direct RNA-seq. Running NanoSim3.0.2 EEF1A1 is on the minus strand.. I would expect a 3'end read bias, but see an increased coverage at the 5'end.

reads did not aligned to 3' end

Top 2 tracks are from NanoSim

RNA model for bonito

Excellent.

RNA model for bonito

BTW, is there any manual to do the training myself ?

RNA model for bonito

Ok, got back to this now.. any developments on this aspect (RNA modifications) @iiSeymour ? I would be happy to do it myself provided that there is some documentation for...

Module to design siRNAs

https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkw1325

Module to design siRNAs

I would like to push this to Version 0.3.0 Do you agree ?