Rosario Nicola Brancaccio
Rosario Nicola Brancaccio
to make the quesion more complete and clear I'll add the format used -task blastn -outfmt "6 qseqid qlen evalue bitscore score pident mismatch gaps ppos staxid ssciname scomname sblastname...
dear developers, First I used Dorado + modbam2bed and I got those not realistic results. Now I tried with Dorado + modkit and I have better results. The problem is...
I have an update. I did a comparison between dorado + modkit and nanopolish for 5mC and I got comparable results. the biggest difference I think is in the threshold...
After a new attempt, I can confirm that Dorado ( like the tools ) produces methylation signals in PCR-amplified samples. The amount of methylation detected is not compatible with background...