B-1991-ing

Dear megabit support team, I already tried "--kmin-1pass" in my command line, why it still assembles from k27 instead of choosing a bigger Kmer? Best, Bing

Thank you for the suggestion, I will rename the MAGs.

Thanks for your confirmation, Matt. I self-checked the number of **scaffolds**, **GC**, **predicted genes** using script.

**"Figure part b Total MAGs unclassi"ed by GTDB-Tk at each taxonomic level."** may not be part of the left sankey figure but only one summary of the total MAG classification...

> ```r > left_annotation3 = rowAnnotation( cell_types=rep(c('Neu', 'Mac','Mono','CMP','DC','B_cell','T','NK'),each = 10), > col = list(cell_type = setNames(brewer.pal(8, "Paired"), unique(colnames(exp_mat))))) > ``` Hi Zuguang, **Why the Heatmap still plot the rows of...

Thank you very much for your reply. Could you please give me your email address?

Update Found one script - https://github.com/zmunro/KrakenTools/tree/master#kraken2_translatepy, which was merged into main fork of KrakenTools (https://github.com/jenniferlu717/KrakenTools) but didn't appear in the downloaded KrakenTools folder. Best, Bing

Update I tried to remove duplicated genes on another big dataset --- 589G. But , finally an error occurred after two hours' running.

Thanks for the reply. Even if I split my 600GB big files, do the dereplication, then combine the duplicated files, the combined file will still need to be dereplicated, but...

Hi Ben, **Update** According to the introduction of **Calculation methods**, Why **the mean coverage of each genome --- 0.02235294** needs to be divided by **the total mean coverage of all...