SprayNPray

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3

Hi, I am using the following call to spray-and-pray.py: ` spray-and-pray.py -t 20 --euk --meta --spades -bam ${prefix}.pairedReads2assembly.sorted.bam -g $file -ref /home/liam/data/nr/nr.dmnd -out spraynpray_out/$bin` I am receiving the following error....

liamfriar

taxmap_slv_ssu_ref_nr_138.1.txt not found

2

So, running the toy dataset and in my own run, I had the same issue. ``` (sprayandpray) [mcallister@poseidon BmuCA187744_spades]$ spray-and-pray.py -g contigs.fasta -ref /data/blastdb/fasta/nr.gz -out sprayNpray_out -t 40 --makedb --spades...

McAllister-NOAA

no blastfile

6

Hi I hope you are well. I am trying to run sprayandpray on a couple of MAGs to decontaminate them. i have installed the program very well and i have...

PercyCapebiosa

fasta file with contigs is not created

3

Hi, Thank you for great tool! I have encountered problem with sprayandpray. When I run: spray-and-pray.py -g assembly.fasta -out endosymbionts_out -cd 0.5 -L 1000000 -perc 50 -ref /BLAST_database/nr_db/nr_diamond_db -Domain Bacteria...

gaworj

universal.tblout not found

6

Hi! I am trying to binning by SprayNPray, and my command is `spray-and-pray.py -g /flash/HusnikU/Cong/assembly/ACAB_assembly.fna -bam /flash/HusnikU/Cong/bam/ACAB.bam -out ACAB -t 32 -ref /apps/unit/BioinfoUgrp/DB/diamondDB/ncbi/238/nr.dmnd --bin` But I got the following error:...

CongLiu37