ribotish
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Feature request: Ribo-seq counts in the results
Thank you for the great tool!
It seems to be working perfectly for me but I would like to request a feature implementation.
Would it be possible to add a column of in-frame Ribo-seq (not TI-seq) count per ORF to the result of ribotish predict
, like TISCount
column for TI-seq?
I believe this would be useful from the following viewpoints (I'm detecting ORFs using Ribo-seq only, without TI-seq):
- The current output includes ORFs with very low read counts. I understand that this is helpful for increasing sensitivity but in some cases users may want to apply filtering by CPM etc to extract highly-translated ORFs.
- With
--framebest
option, one stop codon can have multiple TIS, whenRiboPvalue
has ties (especially whenRiboPvalue
= 0). One option for filtering is taking the longest ORF but it would be more helpful if users can take P-site read abundance into consideration to pick up the ORF with highest translation activity.
I would appreciate it if you could consider implementing this feature, only if it would not cause too much trouble for you. Many thanks, Sotta