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Feature request: Ribo-seq counts in the results

Open nsotta opened this issue 3 years ago • 2 comments

Thank you for the great tool! It seems to be working perfectly for me but I would like to request a feature implementation. Would it be possible to add a column of in-frame Ribo-seq (not TI-seq) count per ORF to the result of ribotish predict, like TISCount column for TI-seq? I believe this would be useful from the following viewpoints (I'm detecting ORFs using Ribo-seq only, without TI-seq):

  1. The current output includes ORFs with very low read counts. I understand that this is helpful for increasing sensitivity but in some cases users may want to apply filtering by CPM etc to extract highly-translated ORFs.
  2. With --framebest option, one stop codon can have multiple TIS, when RiboPvalue has ties (especially when RiboPvalue = 0). One option for filtering is taking the longest ORF but it would be more helpful if users can take P-site read abundance into consideration to pick up the ORF with highest translation activity.

I would appreciate it if you could consider implementing this feature, only if it would not cause too much trouble for you. Many thanks, Sotta

nsotta avatar Nov 09 '20 00:11 nsotta