Error Loading Shared Libraries

[Tj-Idowu]

I am having some problems running the command given to test PEPPAN: PEPPAN -p examples/ST131 -P examples/GCF_000010485.combined.gff.gz examples/*.gff.gz I get an error message:

2021-06-25 15:52:46.357579 COMMAND: /home/idowuol/anaconda3/bin/PEPPAN -p examples/ST131 -P examples/GCF_000010485.combined.gff.gz examples/GCF_000010485.combined.gff.gz examples/GCF_000214765.combined.gff.gz examples/GCF_001566635.combined.gff.gz examples/GCF_001577325.combined.gff.gz 2021-06-25 15:52:48.493142 Run MMSeqs linclust to get exemplar sequences. Params: 0.9 identities and 0.8 align ratio 2021-06-25 15:53:15.976301 Iterative clustering. 8399 exemplars left with identity = 1.0 2021-06-25 15:53:46.618473 Iterative clustering. 6902 exemplars left with identity = 0.99 2021-06-25 15:54:01.761707 Iterative clustering. 6562 exemplars left with identity = 0.98 2021-06-25 15:54:26.676935 Iterative clustering. 6358 exemplars left with identity = 0.97 2021-06-25 15:54:55.481045 Iterative clustering. 6217 exemplars left with identity = 0.96 2021-06-25 15:55:14.961419 Iterative clustering. 6142 exemplars left with identity = 0.95 2021-06-25 15:55:36.852134 Iterative clustering. 6097 exemplars left with identity = 0.94 2021-06-25 15:55:47.590999 Iterative clustering. 6069 exemplars left with identity = 0.93 2021-06-25 15:55:56.188706 Iterative clustering. 6040 exemplars left with identity = 0.92 2021-06-25 15:56:09.833715 Iterative clustering. 6016 exemplars left with identity = 0.91 2021-06-25 15:56:20.936632 Iterative clustering. 5995 exemplars left with identity = 0.9 2021-06-25 15:56:20.988668 Run BLASTn starts /home/idowuol/anaconda3/bin/blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory /home/idowuol/anaconda3/bin/blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory /home/idowuol/anaconda3/bin/blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory /home/idowuol/anaconda3/bin/blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory /home/idowuol/anaconda3/bin/blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory /home/idowuol/anaconda3/bin/blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory /home/idowuol/anaconda3/bin/blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory multiprocessing.pool.RemoteTraceback: """ Traceback (most recent call last): File "/home/idowuol/anaconda3/lib/python3.8/multiprocessing/pool.py", line 125, in worker result = (True, func(*args, **kwds)) File "/home/idowuol/anaconda3/lib/python3.8/site-packages/PEPPAN/modules/uberBlast.py", line 297, in poolBlast if os.path.getsize(outfile) > 0 : File "/home/idowuol/anaconda3/lib/python3.8/genericpath.py", line 50, in getsize return os.stat(filename).st_size FileNotFoundError: [Errno 2] No such file or directory: './NS_hhnlxmnm/qryNA.1.bsn' """

The above exception was the direct cause of the following exception:

Traceback (most recent call last): File "/home/idowuol/anaconda3/lib/python3.8/site-packages/PEPPAN/modules/uberBlast.py", line 345, in run blastab.append(tools[method.lower()](ref, qry)) File "/home/idowuol/anaconda3/lib/python3.8/site-packages/PEPPAN/modules/uberBlast.py", line 500, in runBlast for r in self.pool.imap_unordered(poolBlast, [ [blastn, refDb, q, self.min_id, self.min_cov, self.min_ratio] for q in qrys ]) : File "/home/idowuol/anaconda3/lib/python3.8/multiprocessing/pool.py", line 868, in next raise value FileNotFoundError: [Errno 2] No such file or directory: './NS_hhnlxmnm/qryNA.1.bsn' None /home/idowuol/anaconda3/bin/blastn: error while loading shared libraries: libnsl.so.1: cannot open shared object file: No such file or directory 2021-06-25 15:56:22.771273 Obtained 0 exemplar gene sequences from examples/ST131.clust.exemplar Traceback (most recent call last): File "/home/idowuol/anaconda3/bin/PEPPAN", line 8, in sys.exit(ortho()) File "/home/idowuol/anaconda3/lib/python3.8/site-packages/PEPPAN/PEPPAN.py", line 1897, in ortho get_global_difference(get_gene_group(params['clust'], params['self_bsn']),
File "/home/idowuol/anaconda3/lib/python3.8/site-packages/PEPPAN/PEPPAN.py", line 1593, in get_gene_group bsn = bsn[bsn.T[2] > 0] IndexError: index 2 is out of bounds for axis 0 with size 0 [idowuol@siprapp01p-mean idowuol]$ cmake -bash: cmake: command not found [idowuol@siprapp01p-mean idowuol]$ cd /home/idowuol/anaconda3/bin/blastn -bash: cd: /home/idowuol/anaconda3/bin/blastn: Not a directory