SCP
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Package conflicts with dependencies
Sorry for re-opening this issue but I am stuck here. If I install SeuratObject 4.1.3 or 4.1.4 I get the error:
library(SCP)
Error: package or namespace load failed for ‘SCP’:
object ‘LayerData<-’ is not exported by 'namespace:SeuratObject'
sessionInfo()
R version 4.3.1 (2023-06-16)
Platform: x86_64-apple-darwin20 (64-bit)
Running under: macOS Ventura 13.6.1
Matrix products: default
BLAS: /System/Library/Frameworks/Accelerate.framework/Versions/A/Frameworks/vecLib.framework/Versions/A/libBLAS.dylib
LAPACK: /Library/Frameworks/R.framework/Versions/4.3-x86_64/Resources/lib/libRlapack.dylib; LAPACK version 3.11.0
locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8
time zone: Europe/Amsterdam
tzcode source: internal
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] readxl_1.4.3 yardstick_1.2.0 workflowsets_1.0.1 workflows_1.1.3
[5] tune_1.1.2 rsample_1.2.0 recipes_1.0.8 parsnip_1.1.1
[9] modeldata_1.2.0 infer_1.0.5 dials_1.2.0 scales_1.3.0
[13] broom_1.0.5 tidymodels_1.1.1 gridExtra_2.3 Nebulosa_1.12.0
[17] tradeSeq_1.16.0 slingshot_2.10.0 TrajectoryUtils_1.10.0 princurve_2.1.6
[21] viridis_0.6.4 viridisLite_0.4.2 monocle3_1.3.4 UCell_2.6.2
[25] stringr_1.5.1 DropletUtils_1.22.0 BiocParallel_1.36.0 scDblFinder_1.16.0
[29] SingleCellExperiment_1.24.0 SummarizedExperiment_1.32.0 GenomicRanges_1.54.1 GenomeInfoDb_1.38.1
[33] MatrixGenerics_1.14.0 matrixStats_1.1.0 harmony_1.1.0 Rcpp_1.0.11
[37] easyGgplot2_1.0.0.9000 purrr_1.0.2 tibble_3.2.1 patchwork_1.1.3
[41] biomaRt_2.58.0 enrichplot_1.22.0 org.Hs.eg.db_3.18.0 AnnotationDbi_1.64.1
[45] IRanges_2.36.0 S4Vectors_0.40.2 Biobase_2.62.0 BiocGenerics_0.48.1
[49] clusterProfiler_4.10.0 magrittr_2.0.3 data.table_1.14.8 scCustomize_1.1.3
[53] SeuratObject_4.1.4 Seurat_4.4.0 dplyr_1.1.4 cli_3.6.1
[57] tidyr_1.3.0 ggplot2_3.4.4
loaded via a namespace (and not attached):
[1] igraph_1.5.1 ica_1.0-3 plotly_4.10.3 scater_1.30.0
[5] rematch2_2.1.2 zlibbioc_1.48.0 tidyselect_1.2.0 bit_4.0.5
[9] GPfit_1.0-8 doParallel_1.0.17 clue_0.3-65 lattice_0.22-5
[13] rjson_0.2.21 blob_1.2.4 S4Arrays_1.2.0 parallel_4.3.1
[17] png_0.1-8 ggplotify_0.1.2 goftest_1.2-3 BiocIO_1.12.0
[21] bluster_1.12.0 BiocNeighbors_1.20.0 lhs_1.1.6 uwot_0.1.16
[25] shadowtext_0.1.2 curl_5.1.0 mime_0.12 tidytree_0.4.5
[29] leiden_0.4.3.1 ComplexHeatmap_2.18.0 stringi_1.8.2 backports_1.4.1
[33] XML_3.99-0.16 lubridate_1.9.3 httpuv_1.6.12 paletteer_1.5.0
[37] rappdirs_0.3.3 splines_4.3.1 RcppRoll_0.3.0 mclust_6.0.1
[41] prodlim_2023.08.28 ggraph_2.1.0 sctransform_0.4.1 ggbeeswarm_0.7.2
[45] DBI_1.1.3 terra_1.7-55 HDF5Array_1.30.0 withr_2.5.2
[49] class_7.3-22 xgboost_1.7.6.1 lmtest_0.9-40 tidygraph_1.2.3
[53] rtracklayer_1.62.0 htmlwidgets_1.6.4 fs_1.6.3 ggrepel_0.9.4
[57] SparseArray_1.2.2 cellranger_1.1.0 reticulate_1.34.0 zoo_1.8-12
[61] XVector_0.42.0 timechange_0.2.0 foreach_1.5.2 fansi_1.0.5
[65] grid_4.3.1 timeDate_4022.108 ggtree_3.10.0 rhdf5_2.46.1
[69] R.oo_1.25.0 irlba_2.3.5.1 ggrastr_1.0.2 gridGraphics_0.5-1
[73] ellipsis_0.3.2 lazyeval_0.2.2 yaml_2.3.7 survival_3.5-7
[77] scattermore_1.2 crayon_1.5.2 RcppAnnoy_0.0.21 RColorBrewer_1.1-3
[81] progressr_0.14.0 tweenr_2.0.2 later_1.3.1 ggridges_0.5.4
[85] codetools_0.2-19 GlobalOptions_0.1.2 KEGGREST_1.42.0 Rtsne_0.16
[89] shape_1.4.6 limma_3.58.1 Rsamtools_2.18.0 filelock_1.0.2
[93] DiceDesign_1.9 pkgconfig_2.0.3 xml2_1.3.6 GenomicAlignments_1.38.0
[97] aplot_0.2.2 spatstat.sparse_3.0-3 ape_5.7-1 xtable_1.8-4
[101] plyr_1.8.9 httr_1.4.7 tools_4.3.1 globals_0.16.2
[105] hardhat_1.3.0 beeswarm_0.4.0 nlme_3.1-164 HDO.db_0.99.1
[109] dbplyr_2.4.0 lme4_1.1-35.1 digest_0.6.33 Matrix_1.6-4
[113] furrr_0.3.1 farver_2.1.1 reshape2_1.4.4 ks_1.14.1
[117] yulab.utils_0.1.0 rpart_4.1.23 glue_1.6.2 cachem_1.0.8
[121] BiocFileCache_2.10.1 polyclip_1.10-6 generics_0.1.3 Biostrings_2.70.1
[125] mvtnorm_1.2-4 parallelly_1.36.0 statmod_1.5.0 R.cache_0.16.0
[129] ScaledMatrix_1.10.0 minqa_1.2.6 pbapply_1.7-2 spam_2.10-0
[133] gson_0.1.0 dqrng_0.3.2 utf8_1.2.4 gower_1.0.1
[137] graphlayouts_1.0.2 shiny_1.8.0 lava_1.7.3 GenomeInfoDbData_1.2.11
[141] R.utils_2.12.3 rhdf5filters_1.14.1 RCurl_1.98-1.13 memoise_2.0.1
[145] pheatmap_1.0.12 R.methodsS3_1.8.2 future_1.33.0 RANN_2.6.1
[149] spatstat.data_3.0-3 rstudioapi_0.15.0 cluster_2.1.6 janitor_2.2.0
[153] spatstat.utils_3.0-4 hms_1.1.3 fitdistrplus_1.1-11 munsell_0.5.0
[157] cowplot_1.1.1 colorspace_2.1-0 rlang_1.1.2 DelayedMatrixStats_1.24.0
[161] sparseMatrixStats_1.14.0 ipred_0.9-14 dotCall64_1.1-1 ggforce_0.4.1
[165] circlize_0.4.15 scuttle_1.12.0 mgcv_1.9-0 iterators_1.0.14
[169] abind_1.4-5 GOSemSim_2.28.0 treeio_1.26.0 Rhdf5lib_1.24.0
[173] bitops_1.0-7 promises_1.2.1 scatterpie_0.2.1 RSQLite_2.3.3
[177] qvalue_2.34.0 fgsea_1.28.0 DelayedArray_0.28.0 GO.db_3.18.0
[181] compiler_4.3.1 forcats_1.0.0 prettyunits_1.2.0 boot_1.3-28.1
[185] beachmat_2.18.0 listenv_0.9.0 edgeR_4.0.2 BiocSingular_1.18.0
[189] tensor_1.5 MASS_7.3-60 progress_1.2.2 spatstat.random_3.2-2
[193] R6_2.5.1 fastmap_1.1.1 fastmatch_1.1-4 vipor_0.4.5
[197] ROCR_1.0-11 rsvd_1.0.5 nnet_7.3-19 gtable_0.3.4
[201] KernSmooth_2.23-22 miniUI_0.1.1.1 deldir_2.0-2 htmltools_0.5.7
[205] bit64_4.0.5 spatstat.explore_3.2-5 lifecycle_1.0.4 ggprism_1.0.4
[209] nloptr_2.0.3 restfulr_0.0.15 vctrs_0.6.5 spatstat.geom_3.2-7
[213] snakecase_0.11.1 DOSE_3.28.1 scran_1.30.0 ggfun_0.1.3
[217] sp_2.1-2 future.apply_1.11.0 pracma_2.4.4 pillar_1.9.0
[221] prismatic_1.1.1 metapod_1.10.0 locfit_1.5-9.8 jsonlite_1.8.8
[225] GetoptLong_1.0.5
If I update SeuratObject to > version 5.0.0 which is where LayerData function is introduced, I get the same error as above when I run CellColorHeatmap()
Use the HVF to calculate distance metric.
Use 387 features to calculate distance.
Detected query data type: log_normalized_counts
Detected reference data type: log_normalized_counts
Calculate similarity...
Use 'raw' method to find neighbors.
Predict cell type...
Error: No cell overlap between new meta data and Seurat object
And solution seem to be SeuratObject 4.1.4 or 4.1.3 but they don't work as I stated above.