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Normalize results by fraction of reads in some MultiQC plots

Open uniqueg opened this issue 2 years ago • 5 comments

Is your feature request related to a problem? Please describe. Some MultiQC plots (e.g., from FastQC, tin-score-calculation, STAR), report absolute numbers of reads rather than fractions of reads. While it is useful to know the sequencing depths of the different samples/libraries, it is perhaps not very informative/helpful to represent these differences in multiple plots, as this makes it hard to compare key metrics across samples, e.g., the fraction of (uniquely) mapped reads across samples. This is especially problematic when these plots are exported and put in publications as static images.

Describe the solution you'd like Have one plot that reports sequencing depths/library sizes for each sample as bar plots. Have all other plots report fractions of reads instead.

Additional context It may not be possible to configure MultiQC in a way that read fractions are visualized instead of read numbers. In that case, it is probably too much of a hassle to implement the desired solution.

uniqueg avatar Nov 16 '21 11:11 uniqueg