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Published 20 hours ago • verified publisher icon ydLiu-HIT

Wrong strand information

Open Shisheng-Huang opened this issue 3 years ago • 0 comments

Hi. I am using deSALT 1.5.6 to align PacBio SMRT CCS reads. I found deSALT added wrong strand information (XS:A:+/-) in result sam file. The strand information should indicate the direction of alignment to the genome. For single end alignment, sam flag 0 should correspond to XS:A:+ and sam flag 16 should correspond to XS:A:-. This error can cause serious problems with downstream analysis.

Shisheng-Huang avatar Dec 23 '21 06:12 Shisheng-Huang