DiTing
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xuechunxu
heatmap generate error
您好，
我在运行diting的最后生成热图时出现如下错误提示：
2022-05-26 13:56:34 [INFO ] [8/12] Merge KEGG annotations*
2022-05-26 13:56:34 [INFO ] Merge KEGG annotations
2022-05-26 13:58:06 [INFO ] [9/12] Merge KEGG annotations and gene relative abundances
2022-05-26 13:58:06 [INFO ] Merge abundance table with kegg table
2022-05-26 13:59:20 [INFO ] Produce table of ko abundance among samples
2022-05-26 13:59:31 [INFO ] A table of ko abundance among samples was produced to diting_result/KEGG_annotation/ko_abundance_among_samples.tab
2022-05-26 13:59:31 [INFO ] [9/12] Build gene families
2022-05-26 13:59:31 [INFO ] Build gene family according to diting_result/KEGG_annotation/ko_abun.txt
2022-05-26 14:01:23 [INFO ] [10/12] Generate hierarchical table*
2022-05-26 14:01:23 [INFO ] [11/12] Calculate abundance of pathways*
2022-05-26 14:01:27 [INFO ] [12/12] Diagrammatic drawing
2022-05-26 14:01:27 [INFO ] Visualize abundance table
/home/zhengpf/.conda/envs/diting-env/bin/scripts/sketch.py:62: MatplotlibDeprecationWarning: normalize=None does not normalize if the sum is less than 1 but this behavior is deprecated since 3.3 until two minor releases later. After the deprecation period the default value will be normalize=True. To prevent normalization pass normalize=False
plt.pie(abundance_nor, colors=colors, startangle=90)
Traceback (most recent call last):
File "/home/zhengpf/.conda/envs/diting-env/bin/diting.py", line 255, in
运行脚本如下： diting.py -r /backup/diting/fq -a /backup/diting/contig_layer -o diting_result -n 40 -m 200 运行到最后可以产生pathways_relative_abundance.tab文件，但是无法生成图片，请问您可以看出是什么问题吗？ 谢谢！
您好，
是不是因为样本数太多了啊？超过24个就不可以了，你可以根据需要合并你的样本，然后使用此命令生成图片：diting.py -vis <pathways_relative_abundance.tab>。
