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Question on handling multimappers
Dear RiboCode developers,
According to the documentation, it is recommended to include outFilterMultimapNmax 1 parameter in STAR alignment to exclude non-unique alignments and reduce noise for downstream analyses.
In case of default outFilterMultimapNmax 10 setting, how does RiboCode handle non-unique alignments? Are they included in P-site estimating and ORF detection? Does RiboCode differentiate between primary and secondary alignment flags when dealing with multi-mapped reads?
Thank you very much!