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ARGs-OAP: Online Analysis Pipeline for Antibiotic Resistance Genes Detection from Metagenomic Data Using an Integrated Structured ARG Database

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hello, how can i get SARG v3.2.1-S nucleic acid database? thank you!

Hi, When my data volume is large, I want to accelerate the alignment process. So I want to ask is it reasonable to use diamond(blastx) instead of blast(blastx) in ARG-OAP...

您好,我最近开始学习您开发的这个软件,有一些问题想向您请教一下。 1. ARGs-OAP的2.3版本我记得第一步就需要上传一个metadata,现在3.2这个版本两步都不自己需要上传metadata了是嘛。 2. 之所以关注metadata,是因为我对于2.3版本上传的metadata最后一列readlength有些疑问,我的宏基因组样本里reads长度并不是一个固定的数值,一个样本里reads短到50,长至100(如图片),像这种情况readlength要怎么填写呢,填写最小值或者平均值?当然3.2版本已经不需要上传metadata了,我这种reads长度不一的样本不影响软件使用吧? 3. 图片里的宏基因组数据是不是需要处理成您示例数据那样,就图片里的数据来说只保留名称和序列两行(@开头的行以及序列行)。 多有打扰,期待您的回复,谢谢! ![ba56b144e1d10dcf5137b3921201433](https://github.com/xinehc/args_oap/assets/128786333/9a7dea47-03b1-49fe-9419-098dd95b7063)

hi, I would like to know what is meaning of "count" and "copy" in the output file 'blastout.filtered.txt'. thank you

Hi~~ I was trying to install args_asp package using conda. But something wrong, error came out and couldn't install successfully. error message is listed below: "Retrieving notices: ...working... done WARNING...

Hello The error message, "CRITICAL: No target sequence remained after merging structure files, no further normalization will be made," indicates that there is an issue related to merging structure files...

有个非常奇怪的现象,使用自定义的核苷酸数据库,流程中是使用单端数据bwa分别比对,然后合并提取,我这边尝试了直接用双端数据比对提取序列,发现两者区别很大。单端提取只有24000条,双端提取9万多条。 ![QQ截图20231218143906](https://github.com/xinehc/args_oap/assets/33180540/400bd142-31b2-47b8-89e8-9ba0dfa10c51)

hello, When I was using args_oap/3.2.4 to create a database, I was prompted to enter the file in an incorrect format. ![image](https://github.com/xinehc/args_oap/assets/125781599/5b85bb8b-04d3-4f87-b8d9-a84e07934f07) But I was able to run the same...

could i use it to analyse my nanopore data?thanks !

I used the protein files provided by bacmet database to build a custom database of args_oap, extracted the GI_number of the protein database fasta file according to the tips of...