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error : predicted ORF based on MAG

Open mengqiyue opened this issue 1 year ago • 1 comments

I want to use binned protein sequences to predict ARG. I downloaded the SARG v3.2.1-L (version for long read annotation) and successfully built it using "args_oap make_db -i Long_subdatabase_V3.2.1/Long_subdatabase/3.SARG_v3.2_20220917_Long_subdatabase.fasta". 6936bfe72a6139aa07489be9f1ec9c0

However, I received an error while running stage_one. I only have one protein sequence file as input, with the suffix .faa. image

When I tried changing it to .fa, it still failed to run. How can I solve this problem? I'm trying to run the first stage as follows: "args_oap stage_one -i input -o output -f fa -t 8 --database Long_subdatabase_V3.2.1/Long_subdatabase/3.SARG_v3.2_20220917_Long_subdatabase.fasta" but I get the error. I would really appreciate if you could explain me what's going on here.

Thanks a lot!

mengqiyue avatar Sep 05 '23 08:09 mengqiyue