xiaoli-dong
Normalization step
Does MetaErg perform any gene abundance normalization before mapping to higher functional Ontologies/pathways? The library size of my metagenomic samples are highly variable and without normalizing there will likely be false positives when I perform statistical tests comparing functional pathways between groups.
No, if you look for usages of the sample2depth and mdepth_values values in the search and calculated values the depth values are just added together for each step.
May you explain how the Abundance (vs count) is calculated for each protein-coding gene function profile?
No, if you look for usages of the
sample2depthandmdepth_valuesvalues in the search and calculated values the depth values are just added together for each step.
I couldnt understand the codes