souporcell
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How to identify which sample is which?
Not really a software issue, but related. So souporcell can identify different samples accurately, I believe. But often I will want to know which of my samples corresponds to a cluster, especially when doing treatments or if there is a disease phenotype. Is there an easy way to match samples with clusters? Part of the appeal of souporcell is not having to have genotype information to cluster, but to identify, you will ultimately need some genotype info, yes? What's a good way to do this. Maybe PCR based assays rather than having to do an entire genotype array? Thoughts?
This is a draft of an addition we will include in our next version of the preprint describing how to use overlapping mixtures to recover which donor is which cluster. I will need to provide some software for this as well.
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But an amplicon assay could also work.