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Published 20 hours ago • verified publisher icon welch-lab

Multi-omic

Open zhangqc723 opened this issue 1 year ago • 1 comments

Hello, Now, I had got the output form 10X Multi-omic (RNA+ATAC) by cellranger-arc, and I want to use velocyto run10x for my RNA data form 10X RNA+ATAC. What should I do? Thanks

zhangqc723 avatar May 30 '23 05:05 zhangqc723

Please follow Velocyto's documentation to run their CLI tool. See http://velocyto.org/velocyto.py/tutorial/cli.html#run10x-run-on-10x-chromium-samples.

If that doesn't work on Multiome, you can also try directly calling Velocyto run http://velocyto.org/velocyto.py/tutorial/cli.html#run-run-on-any-technique-advanced-use using the 10X barcode file for --bcfile argument and gex_possorted_bam.bam as the input BAM file.

danielee0707 avatar May 31 '23 19:05 danielee0707