William E. Allen

I realized that the variability was mostly actually due to where the reference electrode was placed relative to the craniotomy, and it always made me nervous positioning the reference wire...

It seemed like it was picking up oscillatory noise when it was resting on the skull further away from the craniotomy and/or there was less saline in the well. This...

How long have you observed the DiI to stick around after doing the recording? I've noticed it will stay there for at least a few days, but I wondered if...

Yeah, if this is useful for people, we built something that holds a beaker of tergazyme right where the mouse's head was, so we don't have to remove the probe....

Good to know :-) That sounds about consistent with my rate... I guess the next question would be: is there some change you made that suddenly caused you to stop...

Yeah, I always remove the constant offset. Good to know, thanks!

I've been using Kilosort + Phy but have recently tried JRCLUST as well. In general, I think the manual cleanup GUI for JRCLUST is much better than Phy -- it...

I guess the other thing that I've noticed is that Phy and Kilosort no longer seem to be under active development, whereas JRCLUST is -- is this true Nick? I've...

@nsteinme Hmm, no I take that into account. In any case, it's not a big deal because I compute it correctly from the raw data anyways. @ajuavinett Does it really...

Good to hear :-) We actually built a neural network system that can discriminate noise vs real neurons with ~99% accuracy (on held out data from a set of ~15...