wajm

hi AnnData object with n_obs × n_vars = 51813 × 30644 obs: 'label', 'label_color' var: 'Selected', 'vst_mean', 'vst_variable', 'vst_variance', 'vst_variance_expected', 'vst_variance_standardized' uns: 'workdir', 'label_color' layers: 'norm_data' but, where are they?

Sorry, I did reciprocal PCA integration. https://satijalab.org/seurat/v3.2/integration.html

okay, I solve the problem. my integration object was changed. DefaultAssay(patient) was RNA. So I return DefaultAssay to "integrated". So sorry and Thank you very much.

one more thing, in case of Importing precomputed seurat object, is it possible to make 3d plot? at the moment I can't.

> > one more thing, > > in case of Importing precomputed seurat object, is it possible to make 3d plot? > > at the moment I can't. > >...

same result with before 2D plot.

> > same result with before 2D plot. > > Sorry but I can't really help much with the current info. I don't know what you mean by 'same result'....

> > > > same result with before 2D plot. > > > > > > > > > Sorry but I can't really help much with the current info....

> > > > > > same result with before 2D plot. > > > > > > > > > > > > > > > Sorry but I...

Same issue here as well