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SAM format screwup

Open calkan opened this issue 6 years ago • 7 comments

after generating SAM with lordfast, trying to convert to BAM:

[W::sam_parse1] mapped query cannot have zero coordinate; treated as unmapped ....

25479 272 22816 0 50 54M * 0 0 TATATCGATATCTACACTCTAGATGCTCATGCTAGTCTCACAGCGACAGCTCAT * AS:i:0 12838 272 47754 0 50 60M * 0 0 CGAGTGATATGCAGTGAGCGAGATCACGCACTGCACAGCTGAGCAGAGCAGACTCATCTC * AS:i:0

and so on. Are you reporting map location 0-based? SAM defines it as 1-based; BAM as 0-based

calkan avatar Dec 18 '17 12:12 calkan