velocyto.py
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Additional gene problem
Hello, I am doing an analysis on data with artificially added genes.
After running the trajectory analysis in Velocyto, when I run scvelo.pl.velocity()
to graph the additional genes, it shows zeros as shown below.
Does anyone have an idea what might be the cause?
Here is some information that might help me think about it. (The gene names are blacked out.)
I wrote a FeaturePlot in Seurat and the additional genes were expressed in sufficient quantities.
The part of the gtf file that corresponds to the additional gene.
Thank you