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Additional gene problem

Open Cadaqoes opened this issue 3 months ago • 0 comments

Hello, I am doing an analysis on data with artificially added genes. After running the trajectory analysis in Velocyto, when I run scvelo.pl.velocity() to graph the additional genes, it shows zeros as shown below. Inkedadd_gene

Does anyone have an idea what might be the cause?

Here is some information that might help me think about it. (The gene names are blacked out.)

I wrote a FeaturePlot in Seurat and the additional genes were expressed in sufficient quantities.

The part of the gtf file that corresponds to the additional gene. add_gene_gtf

Thank you

Cadaqoes avatar May 15 '24 13:05 Cadaqoes