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velocyto on non-UMI bam -U flag

Open anafilia opened this issue 2 years ago • 2 comments

I am trying to run velocyto run on a non-UMI bam file aligned using STAR. I am using -U flag, however velocyto still tries to find cell and UMI codes and I get the following error: OSError: The bam file does not contain cell and umi barcodes appropriately formatted. If you are running UMI-less data you should use the -U flag.

Command: velocyto run -o $output -m $rmsk -U bam.bam $gtf

anafilia avatar Oct 17 '22 08:10 anafilia

I am experiencing same issue

tuhulab avatar May 16 '23 09:05 tuhulab

Same here. Version 0.17.17

Jiayi-Zheng avatar May 13 '24 03:05 Jiayi-Zheng