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Doubt about velocyto run10x count exon and intron matrix how to deal with repeat barcode&UMI

Open seqyuan opened this issue 3 years ago • 0 comments

as the velocyto run10x read the possorted_genome_bam.bam, but I found the bam file not exactly sortted by barcode and UMI, here is an example of possorted_genome_bam.bam:

image

I think if the reads have the same barcode&UMI, it should not be repeat count.

seqyuan avatar May 14 '21 08:05 seqyuan