velocyto.py
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Genes filtered during velocyto running
Hi, when I try to run velocyto --run smartseq or --run, I found that some genes are filtered during this process. The loom file generated, when read by scanpy, contains no mitochondria genes. Also, when I use Starsolo 2.7.6a to perform velocyto, the mitochondria genes are not filtered, but the unspliced transcripts extremely exceed that of spliced ones and even the Gene/raw, which is wierd. Can anyone help me with this problem? thanks