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How can I merge multiple loom files originated from a single bam file

Open tqh003 opened this issue 4 years ago • 0 comments

Hi, Due to memory limitation at the final step of loom generation, As suggested from # 211, I splitted my original bam file (about 64 GB) into Chromosome (chr1-19, X, Y, and MT) using samtools in a loop (script modified from here), and then used velocyto run to get 22 seperate loom files.

I tried loompy.combine to merge these 22 loom files, but it treated different individual chromosome loom files as different samples.

How can I put all the loom files that originated from a single bam (a single sample) together into a single loom file?

Thank you in advance.

tqh003 avatar Feb 15 '21 20:02 tqh003