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Help with different vectorfield figures in R and python
Hi,
I am writing to seek your help with an issue I have with generating RNA velocity figures in R and python - the direction of the arrows don't seem to be consistent between R and python. Can you please let me know it this is expected, and if there's a private link to share my code and data?
The values for parameters I am using in python and R are:
python:
vlm.score_cluster_expression(min_avg_U=0.05, min_avg_S=0.5)
k=20
R: fit.quantile <- 0.02;
kCells = 20
emat <- filter.genes.by.cluster.expression(emat,cell.colors,min.max.cluster.average = 0.5)
nmat <- filter.genes.by.cluster.expression(nmat,cell.colors,min.max.cluster.average = 0.05)
Appreciate all the help.
Thanks Sharvari