Having trouble installing HIV trace

[liusu0825]

Dear Steven, hello! An analysis using the HIV trace tool has recently been needed. However, I'm having some difficulties trying to install the tool and would love your help. I'm using the windows OS and have tried the following steps to install HIV trace: make sure the Python environment and related dependency libraries) are installed. I tried to install HIV trace using the pip3 command: pip3 install hivtrace but the system prompts This error originates from a subprocess, and is likely not a problem with pip. I have consulted relevant documentation and online resources, but I don't seem to find a clear solution to this problem. So I hope you can provide some guidance or advice to help me solve my problem with installing HIV trace. Attached is the error message I am getting during installation: (.venv) PS C:\Users\10920\Desktop\代码\pythonProject> pip3 install biopython Requirement already satisfied: biopython in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-pkages (1.84) Requirement already satisfied: numpy in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-packas (from biopython) (2.1.0) (.venv) PS C:\Users\10920\Desktop\代码\pythonProject> pip3 install numpy Requirement already satisfied: numpy in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-packages (2.1.0) (.venv) PS C:\Users\10920\Desktop\代码\pythonProject> pip3 install scipy Requirement already satisfied: scipy in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-packages (1.14.1) Requirement already satisfied: numpy<2.3,>=1.23.5 in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-packages (from scipy) (2.1.0) (.venv) PS C:\Users\10920\Desktop\代码\pythonProject> pip3 install cython Requirement already satisfied: cython in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-packages (3.0.11) (.venv) PS C:\Users\10920\Desktop\代码\pythonProject> pip3 install hivtrace Collecting hivtrace Using cached hivtrace-0.8.0.tar.gz (39.8 MB) Installing build dependencies ... done Getting requirements to build wheel ... done Preparing metadata (pyproject.toml) ... done Requirement already satisfied: biopython>=1.58 in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-packages (from hivtrace) (1.84) Collecting bioext>=0.21.0 (from hivtrace) Using cached bioext-0.21.8.tar.gz (2.3 MB) Installing build dependencies ... done Getting requirements to build wheel ... done Preparing metadata (pyproject.toml) ... done Collecting hppy>=0.9.9 (from hivtrace) Using cached hppy-0.9.9.tar.gz (5.9 kB) Installing build dependencies ... done Getting requirements to build wheel ... done Preparing metadata (pyproject.toml) ... done Collecting tornado>=4.3 (from hivtrace) Using cached tornado-6.4.1-cp38-abi3-win_amd64.whl.metadata (2.6 kB) Collecting hivclustering>=1.6.8 (from hivclustering[edgefiltering]>=1.6.8->hivtrace) Using cached hivclustering-1.7.0-py3-none-any.whl Requirement already satisfied: numpy>=2.0 in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-packages (from bioext>=0.21.0->hivtrace) (2.1.0) Requirement already satisfied: scipy>=0.15 in c:\users\10920\desktop\代码\pythonproject.venv\lib\site-packages (from bioext>=0.21.0->hivtrace) (1.14.1) Collecting pysam>=0.17 (from bioext>=0.21.0->hivtrace) Using cached pysam-0.22.1.tar.gz (4.6 MB) Installing build dependencies ... done Getting requirements to build wheel ... error error: subprocess-exited-with-error

× Getting requirements to build wheel did not run successfully. │ exit code: 1 ╰─> [40 lines of output] # pysam: cython is available - using cythonize if necessary # pysam: htslib mode is shared # pysam: HTSLIB_CONFIGURE_OPTIONS=None '.' 不是内部或外部命令，也不是可运行的程序 或批处理文件。 '.' 不是内部或外部命令，也不是可运行的程序 或批处理文件。 # pysam: htslib configure options: None Traceback (most recent call last): File "C:\Users\10920\Desktop\代码\pythonProject.venv\Lib\site-packages\pip_vendor\pyproject_hooks_in_process_in_process.py", line 353, in main() File "C:\Users\10920\Desktop\代码\pythonProject.venv\Lib\site-packages\pip_vendor\pyproject_hooks_in_process_in_process.py", line 335, in main json_out['return_val'] = hook(**hook_input['kwargs']) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\10920\Desktop\代码\pythonProject.venv\Lib\site-packages\pip_vendor\pyproject_hooks_in_process_in_process.py", line 118, in get_requires_for_build_wheel return hook(config_settings) ^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\10920\AppData\Local\Temp\pip-build-env-1_bkt2av\overlay\Lib\site-packages\setuptools\build_meta.py", line 332, in get_requires_for_build_wheel return self._get_build_requires(config_settings, requirements=[]) ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\10920\AppData\Local\Temp\pip-build-env-1_bkt2av\overlay\Lib\site-packages\setuptools\build_meta.py", line 302, in _get_build_requires self.run_setup() File "C:\Users\10920\AppData\Local\Temp\pip-build-env-1_bkt2av\overlay\Lib\site-packages\setuptools\build_meta.py", line 502, in run_setup super().run_setup(setup_script=setup_script) File "C:\Users\10920\AppData\Local\Temp\pip-build-env-1_bkt2av\overlay\Lib\site-packages\setuptools\build_meta.py", line 318, in run_setup exec(code, locals()) File "", line 437, in File "", line 81, in run_make_print_config File "C:\Users\10920\AppData\Local\Programs\Python\Python312\Lib\subprocess.py", line 466, in check_output return run(*popenargs, stdout=PIPE, timeout=timeout, check=True, ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\10920\AppData\Local\Programs\Python\Python312\Lib\subprocess.py", line 548, in run with Popen(*popenargs, **kwargs) as process: ^^^^^^^^^^^^^^^^^^^^^^^^^^^ File "C:\Users\10920\AppData\Local\Programs\Python\Python312\Lib\subprocess.py", line 1026, in init self._execute_child(args, executable, preexec_fn, close_fds, File "C:\Users\10920\AppData\Local\Programs\Python\Python312\Lib\subprocess.py", line 1538, in _execute_child hp, ht, pid, tid = _winapi.CreateProcess(executable, args, ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ FileNotFoundError: [WinError 2] 系统找不到指定的文件。 [end of output]

note: This error originates from a subprocess, and is likely not a problem with pip. error: subprocess-exited-with-error

× Getting requirements to build wheel did not run successfully. │ exit code: 1 ╰─> See above for output.

note: This error originates from a subprocess, and is likely not a problem with pip.

Please feel free to let me know if I need to provide more information or do specific actions. Thank you so much for reading this request for help despite your busy schedule and look forward to hearing from you. Good wishes.