DiaNN

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Library contains 0 proteins, and 0 genes

4

Hi @vdemichev, Hi my log shows the following: [0:00] Loading spectral library phl004_canonical_sall_osw.csv WARNING: no neutral loss information found in the library - assuming fragments without losses [0:06] Finding proteotypic...

liv-acollins

Error with DiaNN when loading QE and Orbitrap Fusion Lumos DIA data (**"ERROR: DIA-NN tried but failed to to load the following files..."**)

1

Hello, I am seeing the following ERROR when trying to run files through DiaNN (1.8.1) on my laptop as well as two workstation PCs. All three had DiaNN 1.8.1 downloaded...

biodruid

Convert speclib to tsv format

3

Hi, I am interested in doing a comparison between two pre-existing libraries. Is there a way to convert library in the speclib format to a readable format such as tsv?

Niveda-S

Multiple processors not currently supported

3

It looks like DIA-NN is currently unable to make full use of systems which have multiple processors. While the multi-threading is working, it is only fully utilising a single socket...

liv-acollins

Some raw data could not be processed

1

Dear Vadim, DIANN is really helpful for our proteomic research. We recently met one problem. For example, in one batch of over 300 continuous sequenced data, 12 of them could...

chenliangyu18

PG.Quantity == PG.Top in 85 % cases

1

Dear Vadim, Thank you for developing diann. It is very useful and we love it! We have a dilution series experiment, so MaxLFQ cannot be used (the samples _will_ change)....

bhagwataditya

Library free search

14

Hi Vadim, I am currently analyzing the dia-PASEF data for serum samples. Following are the steps I used to do the analysis. 1. I builded the spectral library using FASTA...

santoshdbhosale

where can I find log file for DIA-NN.

2

DIA-NN seems to be stopping and exiting after running for a few hours. I cant find the log as the GUI exits.

mkarathi

Cannot perform mass calibration

1

Hi Vadim, we recently had some a few incidents where DIA-NN appears to take a very long time "Processing...". When the mass and MS1 accuracy are set to 10ppm then...

haianhle

Help: ERROR: C:\diann\src\diann.cpp: 19135: Cannot load spectral library

1

run fragpipe,stopped for the error. 

shujingpaopao