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DIA-NN - a universal automated software suite for DIA proteomics data analysis.

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Hello Vadim and Team, I am using a spectral library for modified histone peptides that I built in skyline. I have some questions regarding using this library... 1. What is...

Hi Vadim and Team, I would like to use the fragment ions for downstream processing. I am using the --export-quant option and that gives me the Fragment.Quant.Raw column. How do...

There are two warnings when I processed my data with DIANN, I set "--semi" in the command window,however it seems doesn't work. The following warnings or errors (in alphabetic order)...

My diann home page cannot display each option correctly in the correct layout, as shown in the figure. ![image](https://github.com/user-attachments/assets/1c8f7ac1-2937-4952-802c-323e4458e71f) The worst thing is that I can't tell whether my "no...

hi,bro.I used DIA-nn for the first time and wanted to process proteomics data, my boss gave me the data in EXCEL, how can I use dia-nn to process data.Please help...

Hi, Vadim, If I want to extract peptide quantification as following, could I also got their protein names? peptides.maxlfq

Hi Vadim, I’ve been running into this issue, raw files acquired from Orbitrap Astral are not loaded by Diann, no matter it’s 1.8 or 1.9 version/1.9.1 of diann. It happens...

Dear all I am seeking your expertise in analyzing my proteomics data. To enhance the coverage of my dataset, I performed Ph fractionation and subsequently injected the fractions separately. As...

Dear Vadim, I would like to ask a very basic question about Protein.Group in dia-NN. Below is an example of the protein groups extracted from the pg_matrix. Protein.Group Protein.Names Genes...

Hello, I encountered an issue as described in the title. Building Spectral Library using DIA-NN v1.9.1 I created a spectral library using DIA-NN 1.9.1 with the following parameters. The output...