RNAseq-workflow
RNAseq-workflow copied to clipboard
sortmernaREF can not be read
sortmerna
--ref $sortmernaREF
--reads results/2_trimmed_output/SRR1374921_trimmed.fq.gz
--aligned results/3_rRNA/aligned/SRR1374921_aligned.fq.gz
--other results/3_rRNA/filtered/SRR1374921_filtered.fq.gz
--fastx
--log
-a 4
-v
when i run abover code. I don't konwn how to fix the error.
[opt_ref:195] ERROR: The file /home/liulei/RNAseq-workflow/sortmerna_db/rRNA_databases/silva-arc-16s-id95.fasta,/home/liulei/RNAseq-workflow/sortmerna_db/index/silva-arc-16s-id95:/home/liulei/RNAseq-workflow/sortmerna_db/rRNA_databases/silva-arc-23s-id98.fasta,/home/liulei/RNAseq-workflow/sortmerna_db/index/silva-arc-23s-id98:/home/liulei/RNAseq-workflow/sortmerna_db/rRNA_databases/silva-bac-16s-id90.fasta,/home/liulei/RNAseq-workflow/sortmerna_db/index/silva-bac-16s-id95:/home/liulei/RNAseq-workflow/sortmerna_db/rRNA_databases/silva-bac-23s-id98.fasta,/home/liulei/RNAseq-workflow/sortmerna_db/index/silva-bac-23s-id98:/home/liulei/RNAseq-workflow/sortmerna_db/rRNA_databases/silva-euk-18s-id95.fasta,/home/liulei/RNAseq-workflow/sortmerna_db/index/silva-euk-18s-id95:/home/liulei/RNAseq-workflow/sortmerna_db/rRNA_databases/silva-euk-28s-id98.fasta,/home/liulei/RNAseq-workflow/sortmerna_db/index/silva-euk-28s-id98 is not an existing/valid absolute or relative path Reference file (FASTA) absolute or relative path.