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"ERROR: Database check failed" with snpEff and possible fix

Open fpusan opened this issue 2 years ago • 5 comments

Thanks for the nice software! Hopefully this is not duplicated. I got this error when trying to run snippy, installed with mamba, on a gbk reference.

WARNING_GENE_NOT_FOUND: Too many 'WARNING_GENE_NOT_FOUND' warnings, no further warnings will be shown.
WARNING_FRAMES_ZERO: All frames are zero! This seems rather odd, please check that 'frame' information in your 'genes' file is accurate.
ERROR: CDS check file '/data/fer/Betsy/mysnps/reference/./ref/cds.fa' not found.
ERROR: Protein check file '/data/fer/Betsy/mysnps/reference/./ref/protein.fa' not found.
ERROR: Database check failed.

Fix was forcing conda to use snpEff version 4 instead of 5, with conda install -c bioconda snpEff=4. Snippy ran to completion and the output seems ok so far.

fpusan avatar Apr 11 '22 19:04 fpusan

Thank you so much for the fix! I just had the exact same problem.

G4ce99 avatar Apr 13 '22 04:04 G4ce99

Thank you so much for fixing this issue.

Ankitapal1995 avatar May 06 '22 11:05 Ankitapal1995

It worked for me as well! Thanks heaps!

anacmj24 avatar May 19 '22 22:05 anacmj24

I found that snpEff=5.0 also works but snpEff=5.1 does NOT

crarlus avatar Jun 17 '22 12:06 crarlus

This seems to have fixed the problem I was having, thanks!

Kiratrea avatar Jul 14 '22 16:07 Kiratrea