prokka
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Missing short genes
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Prodigal penalizes predicting genes that are shorter than 250 bp (83 aa). As a result, Prokka is missing a number of short proteins that do exist in a closely related species. Any thoughts on how to deal with this? By checking with prodigal -s I've learned that Prodigal is in fact predicting the genes, but they have a score < 0 and so are discarded.