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About SK1 transcriptome reference and m6A sites for Yeast
Hi, I hava two problem in learning nanocompore.
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The following files are provided in https://www.yeastgenome.org/strain/SK1 about reference file site mentioned in paper. Is the
SK1_NCSL00000000_SGD_rna_genomic.fsa
correct file for alignment ? Could you guide me on which Yeast SK1 transcriptome reference file to use for direct yeast RNA ? -
In addition, Nanocompare compiled an orthogonal reference set of m6A sites from SK1 yeast by taking m6ASeq sites from Schwartz et al. and
MAZTER-seq
sites from Garcia-Camposet al. for benchmarking results against known yeast m6A sites. Is it possible to detail the processing of 1297 reference m6A positions? I was unable to successfully reproduce as I can't find the sites file of MAZTER-seq clearly. Could the ground-truth m6a sites set file be shared please? Is there any way can obtain it?
Thanks very much! Best, xu