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About SK1 transcriptome reference and m6A sites for Yeast

Open Derryxu opened this issue 1 year ago • 1 comments

Hi, I hava two problem in learning nanocompore.

  1. The following files are provided in https://www.yeastgenome.org/strain/SK1 about reference file site mentioned in paper. Is the SK1_NCSL00000000_SGD_rna_genomic.fsa correct file for alignment ? Could you guide me on which Yeast SK1 transcriptome reference file to use for direct yeast RNA ? image

  2. In addition, Nanocompare compiled an orthogonal reference set of m6A sites from SK1 yeast by taking m6ASeq sites from Schwartz et al. and MAZTER-seq sites from Garcia-Camposet al. for benchmarking results against known yeast m6A sites. Is it possible to detail the processing of 1297 reference m6A positions? I was unable to successfully reproduce as I can't find the sites file of MAZTER-seq clearly. Could the ground-truth m6a sites set file be shared please? Is there any way can obtain it?

Thanks very much! Best, xu

Derryxu avatar Jul 20 '22 13:07 Derryxu