error in biobakery_workflow_database

[Muneer111]

Hi@abmiguez in hope you are fine, I am trying to install biobakery an i am getting this error tried my ways but failed.followed some tutorials and guides also kindly help and mention the command I will be running in my case like you did it here # Problem 1 biobakery_workflows_databases --install wmgx Installing humann utility mapping database Download URL: http://huttenhower.sph.harvard.edu/humann2_data/full_mapping_v201901.tar.gz Downloading file of size: 2.55 GB

2.55 GB 100.00 % 1.49 MB/sec 0 min -0 sec
Extracting: /home/qiime2/biobakery_workflows_databases/humann/full_mapping_v201901.tar.gz

Database installed: /home/qiime2/biobakery_workflows_databases/humann/utility_mapping

HUMAnN configuration file updated: database_folders : utility_mapping = /home/qiime2/biobakery_workflows_databases/humann/utility_mapping Generating strainphlan fasta database Could not locate a Bowtie index corresponding to basename "/home/qiime2/miniconda/envs/kneaddata/lib/python3.8/site-packages/metaphlan/metaphlan_databases/mpa_v30_CHOCOPhlAn_201901" Error: Encountered internal Bowtie 2 exception (#1) Command: /home/qiime2/miniconda/envs/abmiguez kneaddata/bin/bowtie2-inspect-s --wrapper basic-0 /home/qiime2/miniconda/envs/kneaddata/lib/python3.8/site-packages/metaphlan/metaphlan_databases/mpa_v30_CHOCOPhlAn_201901 Unable to install database. Error running command: bowtie2-inspect /home/qiime2/miniconda/envs/kneaddata/lib/python3.8/site-packages/metaphlan/metaphlan_databases/mpa_v30_CHOCOPhlAn_201901 > /home/qiime2/biobakery_workflows_databases/strainphlan_db_markers/all_markers.fasta

Problem 2 And secondly If hopefully I am done with installation, please let me know the tutorial I will following for analysis my shotgun datsets