Synat Keam

Single cell TCRSeq

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## ❓ Questions and Help We have a set of [listed tutorials available on the website](https://immunarch.com/articles/). Dear Authors, Thanks for creating immunarch. I have 10X single cell TCRseq data 9...

Dear Authors, Hope you are well. First thank you so much for building monocle R packages. I have just started to use monocle3 and have generated the graph as attached....

Script for doublet finder with seurat 5

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Dear Chris and team, Sorry for asking you and your team again. Hope you do not mind. You said Doublet Finder should work with Seurat 5 now. I am using...

Add metadata to seurat obj

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Dear all, It is a bit of coding issue. I have Seurat obj seurat.obj.T and in the metadata, a column type contains three levels of arthritis " First arthritis", "Second...

filter the most significant ligand and receptors in netVisual_bubble

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Dear professor sqjin, I run ```netVisual_bubble``` and ```netVisual_chord_gene``` functions of cellchat to visualize cell communication, but got too many receptor/ligand interaction. I still would like to cover all signalling pathways,...

Could not initialize SCENIC

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Dear Developers, Thanks for developing SCENIC. I apologized in advance as this could be a bit repetitive, but I could not solve it after trying all my best and read...

Dear Authors and professors! Hope you are well and thank you for creating ClusterProfiler package. I am wondering whether you could guide with plotting gseaplots using DEG from Seurat output....

how to read graph_test output of monocle 3

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Dear All, I am using monocle 3 to perform Trajectory analysis and use ```deg