scrublet
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Detect doublets in single-cell RNA-seq data
Dear managers of scrublet, Thank you for your time. I will try to be concise. The gist is, I am curious, with my limited knowledge, what other intepretations or hypotheses...
Hi @swolock , I have a experiment with mixed human and mouse (equal proportion) scRNA-seq. Based on guideline from 10x, the I have provided the expected_doublet_rate as 6.4% expected_doublet_rate=0.064 I...
Hi! I am trying to install a bunch of packages for single cell data analysis, multiome. I am hoping I can also use scrublet, but I am hoping for a...
HI I have recently started using scrublet. Thanks for making it availabel for scientific community. Scrublet worked fine for me for some of the snRNA-seq samples but recently it started...
Hi, I'm trying to run scrublet on some 10x GEX data. I have already run SoupX to correct for ambient RNA. Now I am running: ``` scrub = scr.Scrublet(adata.X, expected_doublet_rate=0.08...
I used scrublet for my sc-rna seq data generated from 10X fixed rna profiling (probe based) and gave me an estimate of 91.7% doublet. That is too high i think,...
I run scrublet on my data and I noticed afterwards that the barcode names had changed. For example, AGAAGCGGTGGTCTGC-1 became AGAAGCGGTGGTCTGC-1-0 and AGAAGCGGTGGTCTGC-1-1 became AGAAGCGGTGGTCTGC-1-1-1. Is that normal? Thank you