Threshold to call doublets

[Jayjay601]

Hi,

Have you seen a similar behavior of picking a threshold using skimage.filters threshold minimum as below before? The threshold automatically set by Scrublet was 0.74 and it seems very high if I looked at the histogram. I just broke the Scrublet threshold calling script down in R to verify the 0.74 threshold. It seems like by eye I could put it around 0.25 instead. Have you seen something similar before? `

sf<-import("skimage.filters") sf$threshold_minimum(scrublet_res$doublet_scores_sim) [1] 0.7433677 hist(scrublet_res$doublet_scores_sim,nclass=100);abline(v=0.7433677) `

Thanks,

J