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Tacox plugin: Sequence mismatches on multiple scaffold on random sequence

Open elija-feigl opened this issue 7 months ago • 0 comments

Affects: tacox plugin: Cadnano design files with multiple scaffold strands, double stranded sections that use the scaffold

Problem:

  • It is only possible to select a single scaffold sequence.
  • either if a single sequence is selected or the random sequence is used the sequences of the other scaffold bases will be completely random, not matching the staple strands (see picture: first scaffold with correct pairs (green), second scaffold with mismatches (red) Screenshot 2024-07-18 at 14 51 17

Expected behaviour: the tacox plugin should not silently create mismatching duplexes.

  • either assign matching random sequences for other then the first scaffold
  • or disallowing designs in the multiple scaffolds altogether

elija-feigl avatar Jul 18 '24 13:07 elija-feigl