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Feature request: trim based on user defined length

Open bwprice opened this issue 4 years ago • 0 comments

It would be really helpful if there was an option to trim the first X and last Y bases as this would really help remove the junk that Sanger seq end up having here, leading to fewer ambiguous bases in the resulting consensus. For example in COI barcoding I find trimming each sequence to 650bp and then trimming the first 100bp before assembly removes 99% of the issues. Assembling the two 550bp sequences still results in a 650bp contig, but with far fewer Ns and clean sequence across the contig, admittedly with 100bp overhangs.

bwprice avatar Oct 05 '21 21:10 bwprice